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saline sodium citrate ssc buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher saline sodium citrate ssc buffer
    Saline Sodium Citrate Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/saline+sodium+citrate/SSC+buffer/pmc13197786-24-0-6
    Average 97 stars, based on 1 article reviews
    saline sodium citrate ssc buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Temporal Stretch‐Induced Nuclear Mechanosensing Coordinates Early Chromatin Accessibility and Genome Protection
    Article Snippet: Treatment with 0.1 m hydrochloric acid (Sigma, H9892) solution for 5 min at room temperature and subsequent triple washing ensued. .. A solution of 10 μg mL −1 RNase A (Thermo Fisher, EN0531) was applied, followed by incubation at 37 °C for 2 h. Following this, samples were washed with 2× Saline Sodium Citrate (SSC; Thermo Fisher, AM9763) and incubated with hybridization buffer (2× SSC with 0.1% Tween (Sigma, P‐7949), 50% formamide (Invitrogen, 15 515 026), 10% dextran sulfate (Merck Millipore, 3730) for 1 h at 47 °C. ..

    Article Title: Temporal Stretch-Induced Nuclear Mechanosensing Coordinates Early Chromatin Accessibility and Genome Protection.
    Article Snippet: Treatment with 0.1 m hydrochloric acid (Sigma, H9892) solution for 5 min at room temperature and subsequent triple washing ensued. .. A solution of 10 μg mL−1 RNase A (Thermo Fisher, EN0531) was applied, followed by incubation at 37 °C for 2 h. Following this, samples were washed with 2× Saline Sodium Citrate (SSC; Thermo Fisher, AM9763) and incubated with hybridization buffer (2× SSC with 0.1% Tween (Sigma, P-7949), 50% formamide (Invitrogen, 15 515 026), 10% dextran sulfate (Merck Millipore, 3730) for 1 h at 47 °C. ..

    Article Title: Cytoplasmic mRNA granules regulate cell fate decisions during PINK1/Parkin mitophagy
    Article Snippet: Cells grown on glass coverslips in 24-well plates were washed with phosphate-buffered saline (PBS; Invitrogen, AM9624), fixed with 4% paraformaldehyde/PBS (Wako, 163-20145) for 15 min at room temperature, and then permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, T8787) for 5 min at 4°C. .. The coverslips were washed with PBS and incubated in hybridization buffer (50% formamide (Sigma-Aldrich, 221198), 1× Denhardt’s solution (Invitrogen, 750018), 1× saline-sodium citrate (SSC; Invitrogen, AM9763), 10 mM ethylenediaminetetraacetic acid (EDTA; Invitrogen, AM9262), 0.01% Tween 20 (Sigma-Aldrich, P9416), and 0.1 mg/ml yeast tRNA (Sigma-Aldrich, R8508)) for 2 h at 55°C. .. An Alexa Fluor 594-conjugated oligonucleotide dT 34 probe (Gene Design, Inc.) in hybridization buffer was added to the coverslips and incubated for 16 h at 55°C.

    Saline:

    Article Title: Temporal Stretch‐Induced Nuclear Mechanosensing Coordinates Early Chromatin Accessibility and Genome Protection
    Article Snippet: Treatment with 0.1 m hydrochloric acid (Sigma, H9892) solution for 5 min at room temperature and subsequent triple washing ensued. .. A solution of 10 μg mL −1 RNase A (Thermo Fisher, EN0531) was applied, followed by incubation at 37 °C for 2 h. Following this, samples were washed with 2× Saline Sodium Citrate (SSC; Thermo Fisher, AM9763) and incubated with hybridization buffer (2× SSC with 0.1% Tween (Sigma, P‐7949), 50% formamide (Invitrogen, 15 515 026), 10% dextran sulfate (Merck Millipore, 3730) for 1 h at 47 °C. ..

    Article Title: Temporal Stretch-Induced Nuclear Mechanosensing Coordinates Early Chromatin Accessibility and Genome Protection.
    Article Snippet: Treatment with 0.1 m hydrochloric acid (Sigma, H9892) solution for 5 min at room temperature and subsequent triple washing ensued. .. A solution of 10 μg mL−1 RNase A (Thermo Fisher, EN0531) was applied, followed by incubation at 37 °C for 2 h. Following this, samples were washed with 2× Saline Sodium Citrate (SSC; Thermo Fisher, AM9763) and incubated with hybridization buffer (2× SSC with 0.1% Tween (Sigma, P-7949), 50% formamide (Invitrogen, 15 515 026), 10% dextran sulfate (Merck Millipore, 3730) for 1 h at 47 °C. ..

    Article Title: Cytoplasmic mRNA granules regulate cell fate decisions during PINK1/Parkin mitophagy
    Article Snippet: Cells grown on glass coverslips in 24-well plates were washed with phosphate-buffered saline (PBS; Invitrogen, AM9624), fixed with 4% paraformaldehyde/PBS (Wako, 163-20145) for 15 min at room temperature, and then permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, T8787) for 5 min at 4°C. .. The coverslips were washed with PBS and incubated in hybridization buffer (50% formamide (Sigma-Aldrich, 221198), 1× Denhardt’s solution (Invitrogen, 750018), 1× saline-sodium citrate (SSC; Invitrogen, AM9763), 10 mM ethylenediaminetetraacetic acid (EDTA; Invitrogen, AM9262), 0.01% Tween 20 (Sigma-Aldrich, P9416), and 0.1 mg/ml yeast tRNA (Sigma-Aldrich, R8508)) for 2 h at 55°C. .. An Alexa Fluor 594-conjugated oligonucleotide dT 34 probe (Gene Design, Inc.) in hybridization buffer was added to the coverslips and incubated for 16 h at 55°C.

    Article Title: Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter
    Article Snippet: .. The cells were then harvested by centrifugation at 6000 RPM for 3 min at room temperature and washed with 0.02% saline sodium citrate (SSC, Invitrogen). .. The cell pellets were resuspended in 300 μl MAAM mix (4:1 V:V dilution of methanol to glacial acetic acid) and incubated at -20 °C for 15 minutes, followed by 1X PBS (from 10X PBS, Invitrogen) wash to remove traces of MAAM.

    Article Title: (p)ppGpp-dependent activation of gene expression during nutrient limitation
    Article Snippet: .. The cells were then harvested by centrifugation at 6,000 RPM for 3 min at room temperature and washed with 0.02% saline sodium citrate (SSC, Invitrogen). .. The cell pellets were resuspended in 300 μL Methanol Acetic Acid Mix (MAAM) (4:1 vol:vol dilution of methanol to glacial acetic acid) and incubated at −20°C for 15 min, followed by 1X PBS (from 10× PBS, Invitrogen) wash to remove traces of MAAM.

    Article Title: Protocol optimization improves the performance of multiplexed RNA imaging
    Article Snippet: .. Briefly, cell culture samples were washed 3 times in 2× saline-sodium citrate (SSC; Thermo, AM9765) and once in 30% (v/v) formamide (Thermo, AM9342) in 2× SSC for 5 min at room temperature. .. Unless otherwise noted, samples were hybridized by incubation in a buffer containing smFISH or MERFISH probes at the reported concentrations suspended in 30% (v/v) deionized formamide, 10% (v/v) dextran sulfate (VWR, 97062-828), and 1 mg/mL yeast tRNA (Thermo, 15401029) in 2× SSC.

    Article Title: (p)ppGpp-dependent activation of gene expression during nutrient limitation.
    Article Snippet: .. The cells were then harvested by centrifugation at 6,000 RPM for 3 min at room temperature and washed with 0.02% saline sodium citrate (SSC, Invitrogen). .. The cell pellets were resuspended in 300 μL Methanol Acetic Acid Mix (MAAM) (4:1 vol:vol dilution of methanol to glacial acetic acid) and incubated at −20°C for 15 min, followed by 1X PBS (from 10× PBS, Invitrogen) wash to remove traces of MAAM.

    Hybridization:

    Article Title: Temporal Stretch‐Induced Nuclear Mechanosensing Coordinates Early Chromatin Accessibility and Genome Protection
    Article Snippet: Treatment with 0.1 m hydrochloric acid (Sigma, H9892) solution for 5 min at room temperature and subsequent triple washing ensued. .. A solution of 10 μg mL −1 RNase A (Thermo Fisher, EN0531) was applied, followed by incubation at 37 °C for 2 h. Following this, samples were washed with 2× Saline Sodium Citrate (SSC; Thermo Fisher, AM9763) and incubated with hybridization buffer (2× SSC with 0.1% Tween (Sigma, P‐7949), 50% formamide (Invitrogen, 15 515 026), 10% dextran sulfate (Merck Millipore, 3730) for 1 h at 47 °C. ..

    Article Title: Temporal Stretch-Induced Nuclear Mechanosensing Coordinates Early Chromatin Accessibility and Genome Protection.
    Article Snippet: Treatment with 0.1 m hydrochloric acid (Sigma, H9892) solution for 5 min at room temperature and subsequent triple washing ensued. .. A solution of 10 μg mL−1 RNase A (Thermo Fisher, EN0531) was applied, followed by incubation at 37 °C for 2 h. Following this, samples were washed with 2× Saline Sodium Citrate (SSC; Thermo Fisher, AM9763) and incubated with hybridization buffer (2× SSC with 0.1% Tween (Sigma, P-7949), 50% formamide (Invitrogen, 15 515 026), 10% dextran sulfate (Merck Millipore, 3730) for 1 h at 47 °C. ..

    Article Title: Cytoplasmic mRNA granules regulate cell fate decisions during PINK1/Parkin mitophagy
    Article Snippet: Cells grown on glass coverslips in 24-well plates were washed with phosphate-buffered saline (PBS; Invitrogen, AM9624), fixed with 4% paraformaldehyde/PBS (Wako, 163-20145) for 15 min at room temperature, and then permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, T8787) for 5 min at 4°C. .. The coverslips were washed with PBS and incubated in hybridization buffer (50% formamide (Sigma-Aldrich, 221198), 1× Denhardt’s solution (Invitrogen, 750018), 1× saline-sodium citrate (SSC; Invitrogen, AM9763), 10 mM ethylenediaminetetraacetic acid (EDTA; Invitrogen, AM9262), 0.01% Tween 20 (Sigma-Aldrich, P9416), and 0.1 mg/ml yeast tRNA (Sigma-Aldrich, R8508)) for 2 h at 55°C. .. An Alexa Fluor 594-conjugated oligonucleotide dT 34 probe (Gene Design, Inc.) in hybridization buffer was added to the coverslips and incubated for 16 h at 55°C.

    Centrifugation:

    Article Title: Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter
    Article Snippet: .. The cells were then harvested by centrifugation at 6000 RPM for 3 min at room temperature and washed with 0.02% saline sodium citrate (SSC, Invitrogen). .. The cell pellets were resuspended in 300 μl MAAM mix (4:1 V:V dilution of methanol to glacial acetic acid) and incubated at -20 °C for 15 minutes, followed by 1X PBS (from 10X PBS, Invitrogen) wash to remove traces of MAAM.

    Article Title: (p)ppGpp-dependent activation of gene expression during nutrient limitation
    Article Snippet: .. The cells were then harvested by centrifugation at 6,000 RPM for 3 min at room temperature and washed with 0.02% saline sodium citrate (SSC, Invitrogen). .. The cell pellets were resuspended in 300 μL Methanol Acetic Acid Mix (MAAM) (4:1 vol:vol dilution of methanol to glacial acetic acid) and incubated at −20°C for 15 min, followed by 1X PBS (from 10× PBS, Invitrogen) wash to remove traces of MAAM.

    Article Title: (p)ppGpp-dependent activation of gene expression during nutrient limitation.
    Article Snippet: .. The cells were then harvested by centrifugation at 6,000 RPM for 3 min at room temperature and washed with 0.02% saline sodium citrate (SSC, Invitrogen). .. The cell pellets were resuspended in 300 μL Methanol Acetic Acid Mix (MAAM) (4:1 vol:vol dilution of methanol to glacial acetic acid) and incubated at −20°C for 15 min, followed by 1X PBS (from 10× PBS, Invitrogen) wash to remove traces of MAAM.

    other:

    Article Title: An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gut
    Article Snippet: Coverslips (Bioptechs 40-1313-03193) were silanized (Sigma 107778) and coated with poly-d-lysine and orange fluorescent beads (Santa Cruz sc-136156; Invitrogen F8800) as previously described , and 10-μm tissue sections were collected on a cryostat (Leica CM1850) set to -23 °C.

    Cell Culture:

    Article Title: Protocol optimization improves the performance of multiplexed RNA imaging
    Article Snippet: .. Briefly, cell culture samples were washed 3 times in 2× saline-sodium citrate (SSC; Thermo, AM9765) and once in 30% (v/v) formamide (Thermo, AM9342) in 2× SSC for 5 min at room temperature. .. Unless otherwise noted, samples were hybridized by incubation in a buffer containing smFISH or MERFISH probes at the reported concentrations suspended in 30% (v/v) deionized formamide, 10% (v/v) dextran sulfate (VWR, 97062-828), and 1 mg/mL yeast tRNA (Thermo, 15401029) in 2× SSC.



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